Composite

Part:BBa_K5036034:Design

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-21)


dCas9(N)_NES-Syn-VEGFR-2 (VEGF-R2, C-TEV, NES, TCS (Q, L), HA, dCas9(N),mCherry)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3352
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 982
    Illegal BglII site found at 2341
    Illegal BglII site found at 3662
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1854
    Illegal BsaI.rc site found at 664
    Illegal BsaI.rc site found at 1442
    Illegal BsaI.rc site found at 2731
    Illegal SapI.rc site found at 3283


Design Notes

NO


Source

ADDGENE

References